Role of mechanosensitive Piezo1 receptor expressed on macrophage in Periodontitis

Abstract Objectives: The mechanosensitive Ca 2+ion channels, Piezo1, converts the mechanical stimulus into cell signals in a variety of immune cells. However, pathophysiological property Piezo1 oral polymicrobial infectious disease periodontitis is largely unknown. This study investigated role inflammatory and phagocytic reactions by RAW264.7 macrophages response to exposures periodontal pathogen, P.gingivalis. Experimental methods: Short hairpin RNA (shRNA) vector for or control scrambled sequence was transfected Then, cells were incubated 24 hours presence absence whole P.gingivalis(MOI; 10) outer membrane vesicles (OMVs, 1 μg/ml) isolated from P.gingivalis, w w/o Yoda1 (synthetic activator), shear stress. qPCR used monitor mRNA expressions TNF-α, TLR-2, TLR-4, whereas TNF-α protein detected ELISA. RAW264.7’s ability evaluated cytoplasm-internalization pHrodo-labeled P.gingivalisor OMVs using flow cytometer. Results: induced more production phagocytosis than both which further upregulated as well stress (p<0.05, ANOVA). Shear combined with OMV-stimulation gene expression while downregulating TLR-2 shRNA-targeting significantly suppressed internalization compared scramble shRNA Conclusion: present indicated that may dysregulate macrophage-mediated innate responses periodontitis. NIH NIDCR grants, DE-027851, DE-028715 DE-029709